Journal: NPJ Biofilms and Microbiomes

Article Title: Engineered Mycoplasma pneumoniae targeting dual-species bacterial biofilms: a novel strategy against infections

doi: 10.1038/s41522-025-00835-2

Figure Lengend Snippet: a Graphical representation of the antibiofilm payloads secreted by M. pneumoniae strain CV8_HAD. b Western blot analysis of CV8_HAD lysate and supernatant (SN) showing expression of the payloads; the CV8 chassis and the loaded ribosomal protein RL7 were used as controls. c Representative images of crystal violet assays used to quantify S. aureus (strain Sa15981) or P. aeruginosa (strain PAO1) biofilms after treating with SN from CV8 strains. CV8_D expresses payload D only; CV8_HA expresses payloads H and A. d Plot showing the quantification of biofilm dissolution by CV8 strains SN treatments in vitro, using the crystal violet assay. Data are shown as the mean of three independent experiments ± SEM. *** p < 0.001, ****p < 0.0001 by one-way Anova followed by the post-hoc Tukey’s multiple comparison tests.

Article Snippet: The attenuated strain CV8 derives from the wild-type M. pneumoniae strain M129 (ATCC 29342, subtype 1), in which genes mpn133 and mpn372 were deleted, and the mpn051 gene was replaced by the gpsA gene from Mycoplasma penetrans .

Techniques: Western Blot, Expressing, Dissolution, In Vitro, Crystal Violet Assay, Comparison